Professional Research Associate - Enzymology (Lampe lab)

Job Summary:

The Lampe Lab is currently recruiting a full-time (100% FTE) Professional Research Associate (PRA) with significant experience in protein enzymology, including: gene cloning, expression and purification, characterization, and enzyme kinetics assay development (e.g., Km, Vmax). The ideal candidate will have specific experience working with bacterial cytochrome P450 (CYP) enzymes and cognate bacterial redox partners (redoxin/reductase pairs), as well as some experience with molecular docking protocols and software, such as AutoDock Vina and UCSF DOCK, and compound library screening. An MS degree or equivalent in Pharmaceutical Science, Biochemistry, Medicinal Chemistry or a closely allied field is required. This is a salaried position for work up to 40 hours per week with full benefits. Working hours are flexible. This position is currently funded for one year, with the possibility of an extension depending on future funding availability.

Key Responsibilities:

• Design and synthesis of bacterial CYP site-directed mutants
• Expression and purification of bacterial CYP, redoxin, and reductase enzymes
• Fluorescent and/or LCMS substrate identification and assay development
• CYP enzyme activity studies
• Michaelis-Menten enzyme kinetics characterization of bacterial CYP enzymes with various substrates
• Identification and characterization of CYP inhibitors via IC50 and Ki determination
• HTS screening of small-molecule libraries for CYP substrates/inhibitors
• Docking and analysis of selected CYP substrates and inhibitors using AutoDock Vina/UCSF DOCK
• Implementation of HTS docking protocols
• Assist student researchers with training and implementation of molecular docking and molecular dynamics software

Work Location:

Onsite - this role is expected to work onsite and is located in Aurora, CO.

Why Join Us:

The University of Colorado Skaggs School of Pharmacy and Pharmaceutical Sciences is nationally recognized for its leadership in pharmacy education and strong commitment to developing and sustaining a diverse faculty, staff and student body. The Department of Clinical Pharmacy provides unprecedented opportunities for individuals with a strong commitment to an academic career encompassing innovative educational, service and scholarly activities.

Why work for the University?

We have AMAZING benefits and offer exceptional amounts of holiday, vacation and sick leave! The University of Colorado offers an excellent benefits package including:

• Medical: Multiple plan options
• Dental: Multiple plan options
• Additional Insurance: Disability, Life, Vision
• Retirement 401(a) Plan: Employer contributes 10% of your gross pay
• Paid Time Off: Accruals over the year
• Vacation Days: 22/year (maximum accrual 352 hours)
• Sick Days: 15/year (unlimited maximum accrual)
• Holiday Days: 10/year
• Tuition Benefit: Employees have access to this benefit on all CU campuses
• ECO Pass: Reduced rate RTD Bus and light rail service

There are many additional perks & programs with the CU Advantage.

Equal Opportunity Statement:

The University of Colorado (CU) is an Equal Opportunity Employer and complies with all applicable federal, state, and local laws governing non-discrimination in employment. We are committed to creating a workplace where all individuals are treated with respect and dignity, and we encourage individuals from all backgrounds to apply, including protected veterans and individuals with disabilities.

Qualifications:

Minimum Qualifications:
Applicants must meet minimum qualifications at the time of hire.

• MS degree or equivalent (>5 years of experience) in Pharmaceutical Science, Biochemistry, Medicinal Chemistry or closely allied field
• Experience with heterologous enzyme expression in E. coli
• Experience with computational docking software and the Linux OS (Ubuntu)

Condition of Employment

• Must be willing and able to work a flexible schedule that may occasionally include evenings/weekends

Preferred Qualifications:

• Experience working with CYP enzymes and CYP enzyme assay development (substrate and co-factor identification)

Knowledge, Skills and Abilities:

• Ability to communicate effectively in English, both in writing and orally.
• Ability to establish and maintain effective working relationships with employees at all levels throughout the institution.
• Ability to complete tasks with minimal supervision
• Ability to multitask, while maintaining outstanding quality of work
• Ability to work independently and productively

How to Apply:

For full consideration, please submit the following document(s):

1. A letter of interest describing relevant job experiences as they relate to listed job qualifications and interest in the position
2. Curriculum vitae / Resume
3. Three professional references including name, address, phone number (mobile number if appropriate), and email address

Applications are accepted electronically ONLY at www.cu.edu/cu-careers.

Questions should be directed to: Dr. Jed Lampe, jed.lampe@cuanschutz.edu

Screening of Applications Begins:

Immediately and continues until position is filled. For best consideration, apply by 7/21/2025.

Anticipated Pay Range:

The starting salary range (or hiring range) for this position has been established as HIRING RANGE:

$58,687 - $60,500 per annum

The above salary range (or hiring range) represents the University's good faith and reasonable estimate of the range of possible compensation at the time of posting. This position is not eligible for overtime compensation unless it is non-exempt.

Your total compensation goes beyond the number on your paycheck. The University of Colorado provides generous leave, health plans and retirement contributions that add to your bottom line.

Total Compensation Calculator: http://www.cu.edu/node/153125

ADA Statement:

The University will provide reasonable accommodations to applicants with disabilities throughout the employment application process. To request an accommodation pursuant to the Americans with Disabilities Act, please contact the Human Resources ADA Coordinator at hr.adacoordinator@ucdenver.edu.

Background Check Statement:

The University of Colorado Anschutz Medical Campus is dedicated to ensuring a safe and secure environment for our faculty, staff, students and visitors. To assist in achieving that goal, we conduct background investigations for all prospective employees.

Vaccination Statement:

CU Anschutz strongly encourages vaccination against the COVID-19 virus and other vaccine preventable diseases. If you work, visit, or volunteer in healthcare facilities or clinics operated by our affiliated hospital or clinical partners or by CU Anschutz, you will be required to comply with the vaccination and medical surveillance policies of the facilities or clinics where you work, visit, or volunteer, respectively. In addition, if you work in certain research areas or perform certain safety sensitive job duties, you must enroll in the occupational health medical surveillance program.